Table of Contents  
RESEARCH LETTER
Year : 2011  |  Volume : 2  |  Issue : 3  |  Page : 202-204  

Anti-inflammatory and analgesic activities of stem bark extracts of Eugenia jambolana


1 P. G. Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta, Karnataka, India
2 Department of Biotechnology, Manipal Life Science Center, Manipal University, Manipal, India

Date of Web Publication29-Jul-2011

Correspondence Address:
Sudeep V Hegde
Department of Biotechnology and Bioinformatics, Jnana Sahyadri campus, Kuvempu University, Shankaraghatta - 577 451, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-500X.83294

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How to cite this article:
Hegde SV, Yarappa RL, Rai PS. Anti-inflammatory and analgesic activities of stem bark extracts of Eugenia jambolana. J Pharmacol Pharmacother 2011;2:202-4

How to cite this URL:
Hegde SV, Yarappa RL, Rai PS. Anti-inflammatory and analgesic activities of stem bark extracts of Eugenia jambolana. J Pharmacol Pharmacother [serial online] 2011 [cited 2019 Nov 18];2:202-4. Available from: http://www.jpharmacol.com/text.asp?2011/2/3/202/83294

Sir,

The world is facing an explosive increase in the incidence of many systemic diseases and hence cost effective complementary therapies are needed. [1] Medicinal plants have been the subject of intense research due to their potential as sources of commercial drugs or as lead compounds in drug development. The drugs which are used presently for the management of pain and inflammatory conditions are either narcotics or non narcotics (NSAIDS), and have known toxic and lethal effects. [2] On the contrary, herbal medicines with good absorption, less toxicity, and easy availability have been used since ancient times. [3] It is therefore,essential that efforts be made to introduce new medicinal plants, to develop cheaper and effective drugs. [4] Eugenia jambolana Lam. (Myrtaceae) has been claimed to reduce different ailments in the folk medicine. We have investigated the anti-inflammatory and analgesic activities of petroleum ether, ethyl acetate, and methanol extracts of E. jambolana stem bark.

The barks of mature plants were collected from the Soraba region of Shimoga district, Karnataka, India, and the taxonomic identification of the plant was done by Dr. Y. L. Ramachandra, Department of Biotechnology, Kuvempu University, Shankaraghatta (Voucher specimen number E/002/2008). Freshly collected whole stem bark of E. jambolana was shade-dried, powdered, and extracted successively in petroleum ether, ethyl acetate, and methanol (LR grade, Merck, India) using the Soxhlet apparatus.

Healthy, inbred male Albino rats of Wistar strain (150 - 200 g) and male Swiss albino mice (25 - 30 g) were used for the study. The experiments were carried out taking appropriate measures to minimize pain or discomfort, in accordance with the guidelines laid down by CPCSEA (INDIA), with regard to the care and use of animals for experimental procedures, and with due clearance from the Institute Animal Ethics Committee. Based on the acute toxicity study, [5] 500 mg/kg, 500 mg/kg, and 200 mg/kg body weight was taken as the therapeutic dose of petroleum ether (EJP), ethyl acetate (EJE), and methanol (EJM) extracts of stem bark of E. jambolana (EJ), respectively.

Acute inflammation was induced by subplantar injection of 0.1 ml of 1% suspension of carrageenan in normal saline, in the right hind paw of the rats, 30 minutes after oral administration of the drugs. The degree of paw volume was measured initially and then at intervals of one, two, three, and four hours after the carrageenan injection, by using the plethysmometer. Diclofenac sodium 5 mg / kg was used as the standard drug. The anti-inflammatory effect of different test extracts was calculated by the following equation:

Anti-inflammatory activity (%) = [(Vc - Vt)/Vc] Χ 100

Where, Vc represents the inflammatory increase in paw volume in the control group, and Vt represents the increase in paw volume in drug-treated animals.

The extracts were tested for analgesic activity using two methods, namely, acetic acid-induced writhing and the tail flick method. The description of the methodology is as follows:

Mice were injected with 10 ml/kg of 3% aqueous acetic acid intraperitoneally (i.p) 30 min after treatment of E. jambolana petroleum ether (EJP), ethyl acetate (EJE), and methanol (EJM) extracts (500, 500, and 200 mg/kg, respectively) or aspirin 30 mg/kg orally, to all groups, as shown in [Table 1]. The number of writhing episodes of an individual mouse was recorded for 20 min after acetic acid treatment.
Table 1: Analgesic activity of different extracts of E. jambolana stem bark on acetic acidinduced writhings in mice

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In the tail flick method, the mice were treated with different crude extracts orally or with pentazocine 25mg/kg i.p. After 30 min the animals were held firmly to immerse its tail in a water bath that was maintained at 54°C. The time required for the violent jerk of the tail, was recorded to assess the response to the noxious stimulus.

The results were expressed as mean ± SEM. The statistical significance was determined by using the Student's t-test and one-way Analysis of Variance (ANOVA), followed by Tukey's multiple pairwise comparison test. The level of significance was considered to be at P < 0.05.

The anti-inflammatory activity of the E. jambolana stem bark extracts were measured against acute paw edema induced by carrageenan and the results are shown in [Figure 1]. The results were comparable to those of diclofenac sodium as a standard anti-inflammatory drug. The extracts of E. jambolana stem bark exhibited a varying degree of activity. The methanol extract (EJM), however, showed a promising result at the dose of 200 mg/kg after four hours, where it caused 30.7% (0.52 ± 0.01) inhibition. Furthermore, the control group did not show any inhibition even after four hours. Carrageenan-induced inflammation is a useful experimental model of acute inflammation for detecting orally active anti-inflammatory agents. Edema formation in the rat paw is a biphasic response. The first phase is mediated through the release of histamine, serotonin, and kinins, whereas, the second phase is due to the release of prostaglandin and slow reacting substances. [6] Similar observations were made in the present investigation where a sudden peak was noticed at three hours. Furthermore, EJM was more effective and comparable to the standard drug diclofenac sodium, which corresponded with the previous findings. [7] The results of our study indicate that E. jambolana is effective against acute inflammatory disorders. The carrageenan-induced inflammation model is a predictive test for anti-inflammatory agents acting against mediators of acute inflammation. [8]

The results of different extracts on acetic acid-induced writhings indicate a significant reduction in writhing with all the test extracts [Table 1]. EJM showed an appreciable percentage of inhibition (56.2 %), which was comparable to the standard drug Aspirin (65.6 %). Moderate activity was observed with EJE (43.4 %) and EJP (40.3 %). The time required for the flicking tail response was found to be delayed in drug-treated animals as compared with the control [Table 2]. EJM (7.7 ± 0.88) was more active with an increase in time for flick response than the EJE (5.75 ± 0.31) and EJP (4.33 ± 0.36), in reducing the intensity of analgesia. The constriction response of the abdomen, induced by acetic acid, is a sensitive method for peripheral analgesic agents. This response was believed to be mediated by the prostaglandin pathways. [9] The interference of the acetic-acid induced writhing response mediated by extracts explored the potential antinociceptive action. However, the reduced number of writhings alone did not provide proof of whether the antinociceptive action was central or peripheral. To understand the mode of action, the extracts were examined using the tail-flick response, which was generally considered a central action. In the tail-flick model, the extracts increased the pain threshold significantly and this indicated the involvement of a higher center. [10] It was through these mechanisms of action that the analgesic activity of E. jambolana bark could be explained.
Table 2: Analgesic activity of different extracts of E. jambolana stem bark on tail fl ick response in mice

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Figure 1: Effect of different extracts of E. jambolana on carrageenaninduced rat paw edema

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The current study presents the analgesic and local anti-inflammatory effects of E. jambolana and justifies the use of this plant by traditional medical practitioners.

 
   References Top

1.Sridhar SB, Sheetal UD, Pai MR, Shastri MS. Preclinical evaluation of the antidiabetic effect of Eugenia Jamolana seed powder in streptozotocin-diabetic rats. Braz J Med Biol Res 2005;38:463-8.   Back to cited text no. 1
    
2.Park JH, Son KH, Kim SW, Chang HW, Bae K, Kang SS, et al. Anti-inflammatory activity of Synurus deltoids. Phytother Res 2004;18:930-3.   Back to cited text no. 2
    
3.Li RW, Mayers SP, Leach DN, Lin GD, Leach G. A cross cultural study: Anti-inflammatory activity of Australian and Chinese plants. J Ethanopharmacol 2003;85:25-32.  Back to cited text no. 3
    
4.Duffy JC, Dearden JC, Rostron C. Design, synthesis and biological testing of a novel series of anti-inflammatory drugs. J Pharm Pharmacol 2001;53:1505-14.  Back to cited text no. 4
    
5.Jalalpure SS, Patil MB, Prakash NS, Hemalatha K, Manvi FV. Hepatoprotective activity of fruits of Piper longum L. Indian J Pharm Sci 2003;65:363-6.   Back to cited text no. 5
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6.Vinegar R, Schreiber W, Hugo R. Biphasic development of carrageenan oedema in rats. J Pharmacol Exp Ther 1969;166:96-103.  Back to cited text no. 6
    
7.Kumar A, Ilavarasan R, Jayachandran T, Deecaraman M, Kumar RM, Aravindan P, et al. Anti-inflammatory activity of Syzygium cumini seed. African J Biotechnol 2008;7:941-3.  Back to cited text no. 7
    
8.Sawadogo WR, Boly R, Lompo M, Some N, Lamien CE, Guissou IP, et al. Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. Int J Pharmacol 2006;2:435-8.  Back to cited text no. 8
    
9.Ribeiro RA, Vale ML, Thomazzi SM, Paschoalato AB, Poole S, Ferreira SH, et al. Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. Eur J Pharmacol 2000;387:111-8.   Back to cited text no. 9
    
10.Whittle BA. The use of changes in capillary permeability in mice to distinguish between narcotic and non-narcotic analgesics. Br J Pharmacol Chemother 1964;22:246-53.  Back to cited text no. 10
    


    Figures

  [Figure 1]
 
 
    Tables

  [Table 1], [Table 2]


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