RESEARCH PAPER
Year : 2012  |  Volume : 3  |  Issue : 1  |  Page : 26-34

Antiproliferative property of n-hexane and chloroform extracts of Anisomeles malabarica (L). R. Br. in HPV16-positive human cervical cancer cells


1 Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
2 Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India; Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, Riyadh, Kingdom of Saudi Arabia
3 National Center for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India
4 Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, Riyadh, Kingdom of Saudi Arabia
5 Mahatma Gandhi Doerenkamp Center for Alternatives to Use of Animals in Life Science Education, Bharathidasan University, Tiruchirappalli,Tamil Nadu, India; Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, Riyadh, Kingdom of Saudi Arabia

Correspondence Address:
Mohammad Abdulkader Akbarsha
Mahatma Gandhi Doerenkamp Center for Alternatives to Use of Animals in Life Science Education, Bharathidasan University, Tiruchirappalli - 620 024, Tamil Nadu, India

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Source of Support: The DST-FIST [grant No. SR/FST/LSI -112/2002] and UGC-SAP [grant No. F. 3 - 5/2007 (SAP- II)], Conflict of Interest: None


DOI: 10.4103/0976-500X.92500

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Objectives: To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive. Materials and Methods: The whole plant was extracted in n-hexane, chloroform, ethyl acetate, n-butanol, methanol, and water. The cells were treated with the extracts at increasing concentrations to find the IC 50 , adopting MTT ([3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]) assay. Acridine orange (AO) and ethidium bromide (EB) and Hoechst 33258 staining were adopted to assess the mode of cell death, Annexin V-Cy3 staining to evaluate one of the early apoptotic features, JC-1 staining to assess the mitochondrial membrane depolarization, comet assay for DNA fragmentation, and cell cycle analysis for the distribution of cells after treatment. Results: n-Hexane and chloroform extracts were cytotoxic to the cervical cancer cells in dose- and duration-dependent manner. The cells that responded to the treatments revealed typical apoptotic features. Early features of apoptosis, phosphatidyl serine translocation and loss of mitochondrial trans-membrane potential, were observed in the treated cells, and comet assay revealed DNA damage. In the FACS analysis, the cells accumulated in the sub-G0/G1 phase of the cell cycle, except in n-hexane- and chloroform extract-treated SiHa cells at 24 h, which showed arrest in S- and G2/M phases. Conclusions: n-Hexane and chloroform extracts of A. malabarica inhibit proliferation of and induce death in HPV16-positive cervical cancer cells, mostly by apoptosis and to some extent by necrosis.


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