RESEARCH PAPER
Year : 2013  |  Volume : 4  |  Issue : 3  |  Page : 198-203

Effects of structural analogues of apelin-12 in acute myocardial infarction in rats


Russian Cardiology Research and Production Complex, Institute of Experimental Cardiology, Moscow, Russian Federation

Correspondence Address:
Oleg I Pisarenko
Russian Cardiology Research and Production Complex, Moscow
Russian Federation
Login to access the Email id

Source of Support: The Russian Foundation for Basic Research No. 11.04.00078a, Conflict of Interest: None


DOI: 10.4103/0976-500X.114600

Rights and Permissions

Objective: To examine cardioprotective effects of Ρ-terminal fragment of adipokine apelin-12 (A12), its novel structural analogue [MeArg1 , NLe 10 ]-A12 (I), and [d-Ala 12 ]-A12 (II), a putative antagonist of APJ receptor, employing in vivo model of ischemia/reperfusion (I/R) injury. Materials and Methods: Peptides were synthesized by the automatic solid phase method using Fmoc technology. Anesthetized open-chest male Wistar rats were subjected to left anterior descending (LAD) coronary artery occlusion and coronary reperfusion. Hemodynamic variables and electrocardiogram (ECG) were monitored throughout the experiment. Myocardial injury was assessed by infarct size (IS), activity of necrosis markers in plasma, and metabolic state of the area at risk (AAR). Results: Intravenous injection of A12, I, or II at the onset of reperfusion led to a transient reduction of the mean arterial pressure. A12 or I administration decreased the percent ratio of IS/AAR by 40% and 30%, respectively, compared with control animals which received saline. Both peptides improved preservation of high-energy phosphates, reduced lactate accumulation in the AAR, and lowered CK-MB and LDH activities in plasma at the end of reperfusion compared with these indices in control. Treatment with II did not significantly affect either the IS/AAR, % ratio, or activities of both markers of necrosis compared with control. The overall metabolic protection of the AAR in the treated groups increased in the following rank: II < A12 < I. Conclusions: The structural analogue of apelin-12 [MeArg 1 , NLe 10 ]-A12 may be a promising basis to create a new drug for the treatment of acute coronary syndrome.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2218    
    Printed76    
    Emailed0    
    PDF Downloaded430    
    Comments [Add]    
    Cited by others 13    

Recommend this journal