Journal of Pharmacology and Pharmacotherapeutics

RESEARCH PAPER
Year
: 2015  |  Volume : 6  |  Issue : 3  |  Page : 147--153

The effects of A2B receptor modulators on vascular endothelial growth factor and nitric oxide axis in chronic cyclosporine nephropathy


Leena Patel1, Aswin Thaker2 
1 Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Anand, Gujarat, India
2 Department of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

Correspondence Address:
Leena Patel
Department of Pharmacology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa - 388 421, Taluka - Petlad, District - Anand, Gujarat
India

Introduction: To investigate the actions of adenosine A2B receptor modulators on VEGF and NO levels in CsA nephropathy. Materials and Methods: Nephropathy was induced by administrating 25 mg/kg (s.c) of CsA for 5 weeks. The VEGF and NO levels were measured in kidney tissue. Serum creatinine, creatinine clearance, urinary albumin excretion, blood urea nitrogen, kidney pathology score were measured to assess renal function. The analysis of mRNA expression of A2B receptor and VEGF was performed. Results: Administration of CsA for 5 weeks induced adverse renal function. The mRNA expression of VEGF was reduced in renal tissue after 5 weeks of CsA treatment. The renal VEGF and NO levels were also reduced in these animals. In vivo administration of A2B adenosine receptor agonist increased renal VEGF which was inhibited by a selective A2BAR antagonist (MRS1754) in CsA-treated animals. The increase in VEGF was associated with reversal of adverse renal functions. The effects of A2BAR modulators were prominent in CsA-treated animals compared with control animals suggesting CsA treatment may upregulate A2BARs. The mRNA expression of A2BAR was increased after 5 weeks of CsA. Conclusions: A2BAR modulators may provide new therapeutic options to retard CsA nephropathy by mediating renal VEGF and NO.


How to cite this article:
Patel L, Thaker A. The effects of A2B receptor modulators on vascular endothelial growth factor and nitric oxide axis in chronic cyclosporine nephropathy.J Pharmacol Pharmacother 2015;6:147-153


How to cite this URL:
Patel L, Thaker A. The effects of A2B receptor modulators on vascular endothelial growth factor and nitric oxide axis in chronic cyclosporine nephropathy. J Pharmacol Pharmacother [serial online] 2015 [cited 2020 Aug 15 ];6:147-153
Available from: http://www.jpharmacol.com/article.asp?issn=0976-500X;year=2015;volume=6;issue=3;spage=147;epage=153;aulast=Patel;type=0