Figure 5: Effects of NPS2390 on the gentamicin-induced [Ca²⁺]i elevation. Gin-1 cells were pretreated with NPS2390 (10 μM) for 10 min prior to the application of gentamicin (500 μM). Upper panel: representative trace shows the effect of gentamicin on [Ca²⁺]i in the absence or presence of NPS2390. Lower panel: effect of NPS2390 on the gentamicin-stimulated Ca²⁺ elevation. Data are mean ± SEM, N = 22. ****P < 0.001 compare to corresponding pretreated values