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  Indian J Med Microbiol
 

Figure 1: High performance thin layer chromatogram (HPTLC) of the active fraction (AF) and active principle isolated from Z. diphylla. (1) Silica gel 60F254 HPTLC using n-hexane: chloroform (1:1) as a solvent system. Individual components were derivatized with anisaldehyde-sulfuric acid spray reagent. (a) HPTLC of active fraction (AF) showing five components (bands). The fifth band with an Rf value of 0.83 is the active compound (principle). (b) HPTLC of active principle ( Rf 0.83) isolated from AF. (2) Silica gel 60F254 HPTLC scanned densitometrically at 580 nm after derivatization. (a) HPTLC of AF showing five components. (b) HPTLC of active principle ( Rf 0.83) isolated from AF

Figure 1: High performance thin layer chromatogram (HPTLC) of the active fraction (AF) and active principle isolated from <i>Z. diphylla.</i> (1) Silica gel 60F<sub>254</sub> HPTLC using <i>n</i>-hexane: chloroform (1:1) as a solvent system. Individual components were derivatized with anisaldehyde-sulfuric acid spray reagent. (a) HPTLC of active fraction (AF) showing five components (bands). The fifth band with an <i>R</i><sub>f</sub> value of 0.83 is the active compound (principle). (b) HPTLC of active principle ( <i>R</i><sub>f</sub> 0.83) isolated from AF. (2) Silica gel 60F<sub>254</sub> HPTLC scanned densitometrically at 580 nm after derivatization. (a) HPTLC of AF showing five components. (b) HPTLC of active principle ( <i>R</i><sub>f</sub> 0.83) isolated from AF