Year : 2011  |  Volume : 2  |  Issue : 1  |  Page : 30-35

Pharmacological evidences for the stimulation of calcium-sensing receptors by nifedipine in gingival fibroblasts

Department of Dental Pharmacology, Matsumoto Dental University, Shiojiri 399-0781, Japan

Correspondence Address:
Toshimi Hattori
1780 Hirooka-Gohbara, Shiojiri
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0976-500X.77111

Rights and Permissions

Objective: To investigate pharmacologically whether CaSRs are involved in the Ca 2+ antagonist-induced [Ca 2+]i elevation in gingival fibroblasts. Materials and Methods: Gin-1 cells, normal human gingival fibroblasts, were used as the material. The [Ca 2+ ]i was measured with fura-2/AM, a Ca 2+ -sensitive fluorescent dye. Results: At first, we confirmed the existence of CaSRs in these cells by showing that [Ca 2+ ]i was elevated by high concentrations of extracellular Ca 2+ and by prototypic agonists of the CaSR such as gentamicin. The action of gentamicin was antagonized by inhibitors of phospholipase C (PLC), inositol trisphosphate (IP 3 ) receptors, NSCCs, and, importantly, by the CaSR antagonist, NPS2390. Furthermore, the action of gentamicin was potentiated by activators of PLC and protein kinase C (PKC). This confirmed the pathway components mediating Ca 2+ responses to a known agonist of the CaSR. We then investigated whether nifedipine (an L-type Ca 2+ channel blocker) stimulates CaSRs to elevate [Ca 2+ ]i via a similar mechanism. Nifedipine Ca 2+ responses were dose-dependently blocked by NPS2390 and by the same inhibitors of PLC, IP 3 receptors, and NSCCs that disrupted the action of gentamicin. Calphostin C (a PKC inhibitor) and TMB-8 (an inhibitor of Ca2+ release from stores) also inhibited the nifedipine-induced [Ca2+ ]i elevation. Conclusion: These findings suggest that CaSRs are involved in the nifedipine-induced [Ca 2+ ]i elevation in gingival fibroblasts.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded539    
    Comments [Add]    
    Cited by others 5    

Recommend this journal