Journal of Pharmacology and Pharmacotherapeutics

RESEARCH PAPER
Year
: 2016  |  Volume : 7  |  Issue : 4  |  Page : 177--181

Inhibition by tadalafil of contractility of isolated nonpregnant human myometrium


Sumalya Sen1, Anitha Thomas2, Saibal Das1, Jayanta Kumar Dey1, Abraham Peedicayil2, Vinotha Thomas2, Jacob Peedicayil1 
1 Department of Pharmacology and Clinical Pharmacology, Christian Medical College, Vellore, Tamil Nadu, India
2 Division of Obstetrics and Gynaecology, Christian Medical College, Vellore, Tamil Nadu, India

Correspondence Address:
Jacob Peedicayil
Department of Pharmacology and Clinical Pharmacology, Christian Medical College, Vellore, Tamil Nadu
India

Objective: To investigate the inhibitory effect of tadalafil on the contractility of isolated nonpregnant human myometrium. Materials and Methods: The ability of tadalafil (25, 40, and 63 μM) to inhibit 55 mM KCl-induced contractility of isolated nonpregnant human myometrium was studied. The ability of the ATP-sensitive potassium channel blocker glibenclamide (10 μM) and the calcium-sensitive potassium channel (BKCa) blocker iberiotoxin (100 nM) to reverse the inhibitory effect of 40 μM tadalafil on 55 mM KCl-induced myometrial contractility was also studied. Results: Tadalafil produced a concentration-dependent inhibition of myometrial contractility that was statistically significant at 40 and 63 μM concentrations of tadalafil. The inhibition by tadalafil of myometrial contractility was statistically significantly reversed by the concurrent administration of glibenclamide and iberiotoxin. Conclusions: These results suggest that tadalafil inhibits human myometrial contractility by opening ATP-sensitive potassium channels and BKCa channels. The opening of these channels could have been due to the action of raised intracellular levels of cGMP due to inhibition of PDE-5 by tadalafil. The results suggest that tadalafil could be investigated for use in clinical conditions requiring relaxation of the myometrium.


How to cite this article:
Sen S, Thomas A, Das S, Dey JK, Peedicayil A, Thomas V, Peedicayil J. Inhibition by tadalafil of contractility of isolated nonpregnant human myometrium.J Pharmacol Pharmacother 2016;7:177-181


How to cite this URL:
Sen S, Thomas A, Das S, Dey JK, Peedicayil A, Thomas V, Peedicayil J. Inhibition by tadalafil of contractility of isolated nonpregnant human myometrium. J Pharmacol Pharmacother [serial online] 2016 [cited 2021 Jun 13 ];7:177-181
Available from: http://www.jpharmacol.com/article.asp?issn=0976-500X;year=2016;volume=7;issue=4;spage=177;epage=181;aulast=Sen;type=0