Journal of Pharmacology and Pharmacotherapeutics

RESEARCH PAPER
Year
: 2020  |  Volume : 11  |  Issue : 3  |  Page : 100--106

Targeting oxidative stress, transforming growth factor beta-1, and the mammalian target of rapamycin by valproic acid to ameliorate bleomycin-induced scleroderma


Maaly A Abd Elmaaboud1, Mohamed S Omar2, Ahmed M Kabel1 
1 Department of Pharmacology, Faculty of Medicine, Tanta University, Tanta, Egypt
2 Department of Chemistry, Faculty of Science, Benha University, Benha, Egypt

Correspondence Address:
Ahmed M Kabel
Pharmacology Department, Faculty of Medicine, Tanta University, Tanta
Egypt

Objective: To explore the possible ameliorative effect of valproic acid on the experimental model of skin fibrosis induced by bleomycin. Materials and Methods: Forty male BALB/c mice were divided into four equal groups as follows: control group, bleomycin group, bleomycin + Valproic acid group, and Valproic acid group. Mice were assessed for their body weight every 3 days throughout the whole study. Skin tissues were used to evaluate the oxidative stress parameters, transforming growth factor beta 1 (TGF-β1), tumor necrosis factor alpha, interleukin 15, and mammalian target of rapamycin (mTOR). Skin fibrosis was evaluated by measuring dermal thickness and staining the skin tissues with Masson trichrome stain. Furthermore, the skin tissues were immunostained with alpha smooth muscle actin (α-SMA). Results: Administration of Valproic acid to bleomycin-treated mice resulted in the restoration of the body weight with significant decrease in the dermal thickness, amelioration of oxidative stress, suppression of TGF-β1 and mTOR expression, and significant reduction of the percentage of α-SMA immunostaining and the proinflammatory cytokine levels compared to mice treated with bleomycin alone. Conclusion: Valproic acid has an antifibrotic effect on skin fibrosis which may represent a beneficial therapeutic modality for the management of scleroderma.


How to cite this article:
Abd Elmaaboud MA, Omar MS, Kabel AM. Targeting oxidative stress, transforming growth factor beta-1, and the mammalian target of rapamycin by valproic acid to ameliorate bleomycin-induced scleroderma.J Pharmacol Pharmacother 2020;11:100-106


How to cite this URL:
Abd Elmaaboud MA, Omar MS, Kabel AM. Targeting oxidative stress, transforming growth factor beta-1, and the mammalian target of rapamycin by valproic acid to ameliorate bleomycin-induced scleroderma. J Pharmacol Pharmacother [serial online] 2020 [cited 2021 Apr 14 ];11:100-106
Available from: http://www.jpharmacol.com/article.asp?issn=0976-500X;year=2020;volume=11;issue=3;spage=100;epage=106;aulast=Abd;type=0