Close
  Indian J Med Microbiol
 

Figure 1: Transient transfection of pMDR1-EGFP in RB cell line: (a) RB cells transfected with MDR1-EGFP in RB cell line showed the expression of P-gp by western blot in RB cell line and the untransfected cells showed no expression of P-gp in RB cell line. (b and c) Effect of curcumin on MDR1 mRNA and protein expression in RB cell line: Dose-dependent effect of curcumin (2, 5, 10 μM) after 72 hours on the MDR1 mRNA and protein expression in Y79-MDR1 cells. Lane 1: Control cells, Lane 2: 2 μM curcumin, Lane 3: 5 μM curcumin, Lane 4: 10 μM curcumin and Lane 5: Molecular weight marker. (d) Densitometric analysis for the MDR1/P-gp expression. For MDR1 mRNA studies, data are expressed as the mean ratio of MDR/GAPDH mRNA band density and P-gp/β-actin band intensity

Figure 1: Transient transfection of pMDR1-EGFP in RB cell line: (a) RB cells transfected with MDR1-EGFP in RB cell line showed the expression of P-gp by western blot in RB cell line and the untransfected cells showed no expression of P-gp in RB cell line. (b and c) Effect of curcumin on MDR1 mRNA and protein expression in RB cell line: Dose-dependent effect of curcumin (2, 5, 10 μM) after 72 hours on the MDR1 mRNA and protein expression in Y79-MDR1 cells. Lane 1: Control cells, Lane 2: 2 μM curcumin, Lane 3: 5 μM curcumin, Lane 4: 10 μM curcumin and Lane 5: Molecular weight marker. (d) Densitometric analysis for the MDR1/P-gp expression. For MDR1 mRNA studies, data are expressed as the mean ratio of MDR/GAPDH mRNA band density and P-gp/β-actin band intensity