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  Indian J Med Microbiol
 

Figure 3: Hepatocyte growth factor (HGF) concentration in the culture medium of MRC-9 cells after incubation with heparin-derived disaccharides at 37°C or after washing cells with disaccharides at 4°C. (a) Experimental design to examine the contribution of HGF release from cells to the increase in HGF levels. MRC-9 cells were incubated with disaccharides at 4°C for 30 min (gray) after preincubation of cells in the absence of disaccharides (white) at 37°C for 24 h compared with incubation in the presence of disaccharides at 37°C for 24 h (black). (b) HGF concentrations in the culture media of MRC-9 cells. NS, NAc(6S) and NS(6S) indicate the disaccharides ΔUA-GlcNS, ΔUA-GlcNAc(6S) and ΔUA-GlcNS(6S), respectively. Disaccharides were used at 50 μg/mL. Heparin (1 μg/mL) was used as a positive control for HGF induction at 37°C for 24 h. Each value is the mean ± SD (n = 4 in each group)

Figure 3: Hepatocyte growth factor (HGF) concentration in the culture medium of MRC-9 cells after incubation with heparin-derived disaccharides at 37°C or after washing cells with disaccharides at 4°C. (a) Experimental design to examine the contribution of HGF release from cells to the increase in HGF levels. MRC-9 cells were incubated with disaccharides at 4°C for 30 min (gray) after preincubation of cells in the absence of disaccharides (white) at 37°C for 24 h compared with incubation in the presence of disaccharides at 37°C for 24 h (black). (b) HGF concentrations in the culture media of MRC-9 cells. NS, NAc(6S) and NS(6S) indicate the disaccharides ΔUA-GlcNS, ΔUA-GlcNAc(6S) and ΔUA-GlcNS(6S), respectively. Disaccharides were used at 50 μg/mL. Heparin (1 μg/mL) was used as a positive control for HGF induction at 37°C for 24 h. Each value is the mean ± SD (<i>n</i> = 4 in each group)